Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: Effect of curcumin on cell proliferation and mean of IC 50 values of K9TCC-PU AXA ( A ), AXC ( B ), and SH ( C ) canine and human T24 ( D ) cell lines after 72 h of incubation. *** p < 0.001 and **** p < 0.0001. Vertical intermittent lines show IC 50 dose and colored dots represent the drug dose (ng/mL).

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: Effect of D6 turmeric on cell proliferation and mean IC 50 values of K9TCC-PU AXA ( A ), AXC ( B ), and SH ( C ) canine and human T24 ( D ) cell lines after 72 h of incubation. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, and ns = not significant. Vertical intermittent lines show IC 50 dose and colored dots represent the drug dose (µL/mL).

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: Effect of mitoxantrone hydrochloride on cell proliferation and mean IC 50 values of K9TCC-PU AXA ( A ), AXC ( B ), and SH ( C ) canine and human T24 ( D ) cell lines after 24 h of incubation. ** p < 0.01, *** p < 0.001, and **** p < 0.001. Vertical intermittent lines show IC 50 dose and colored dots represent the drug dose (µM).

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: Annexin V-FITC/PI after 72 h of incubation with curcumin in canine AXA, AXC, and SH, and human T24 tumor cells. The upper left quadrant (yellow) represents the group of necrotic cells, and the one on the upper right (blue), the group of cells in late apoptosis. The cells in the lower right quadrant (green) represent the group of cells in early apoptosis and the lower left quadrant (pink), the viable cells.

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Antitumor Effect of Curcumin, D6 Turmeric, and Hydrochloride Mitoxantrone on Canine and Human Urothelial Cancer Cells

doi: 10.3390/ani15111589

Figure Lengend Snippet: The cell migration assay of human urothelial bladder carcinoma cells (T24) treated with curcumin, D6 turmeric, and mitoxantrone hydrochloride and their respective vehicles. The bar graphs represent the mean account of cells in four regions of each well randomly ( left ). The representative microscopy images of the migration assay show T24 cells treated with compounds or vehicles for 24 h, Giemsa, obj 200× ( right ). *** p < 0.001; **** p < 0.0001, and ns = not significant.

Article Snippet: The human T24 bladder carcinoma cell line (ATCC HTB-4TM), donated by Dr. Fernanda Bueno Morrone (Pontifical Catholic University of Rio Grande do Sul, Brazil), originated from a grade III invasive tumor from an 81-year-old female patient [ ].

Techniques: Cell Migration Assay, Microscopy, Migration

Journal: Oncology Letters

Article Title: USP5 promotes tumor progression by stabilizing SLUG in bladder cancer

doi: 10.3892/ol.2024.14705

Figure Lengend Snippet: USP5 expression is associated with tumor progression in patients with bladder cancer. (A) Relationship between the USP5 expression level and overall survival using data from TCGA-BLCA dataset. Analysis of USP5 expression in different (B) T and (C) N stages using data from TCGA-BLCA dataset. (D) Correlation analysis of USP5 with epithelial-mesenchymal transition markers (CDH2, ACAT2 and VIM) using data from TCGA-BLCA dataset. (E) Representative immunohistochemistry staining images of USP5 in normal bladder tissue, NMIBC and MIBC. *P<0.05. USP5, ubiquitin-specific proteinase 5; TCGA-BLCA, The Cancer Genome Atlas-Bladder cancer; T, tumor; N, node; CDH2, cadherin 2; ACAT2, acetyl-CoA acetyltransferase 2; VIM, vimentin; NMIBC, non-basal invasive bladder cancer; MICB, muscle invasive bladder cancer; TPM, transcripts per million; HR, hazard ratio; CI, confidence interval.

Article Snippet: The human bladder urothelial carcinoma T24 cell line was purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Expressing, Immunohistochemistry, Staining, Ubiquitin Proteomics

Journal: Oncology Letters

Article Title: USP5 promotes tumor progression by stabilizing SLUG in bladder cancer

doi: 10.3892/ol.2024.14705

Figure Lengend Snippet: USP5 promotes the malignant phenotype of human bladder cancer T24 cells. (A) Protein expression levels of USP5 in knockdown and overexpression human bladder cancer T24 cell lines. (B) Cell scratch test results of human bladder cancer T24 cells after knockdown and overexpression of USP5. Images were captured with a light microscope at 0 and 24 h. Results of the (C) Transwell and (D) cell proliferation assays after the knockdown and overexpression of USP5. (E) Western blot results demonstrate the expression of epithelial-mesenchymal transition-related proteins (E-cadherin, N-cadherin and vimentin) in human bladder cancer T24 cells after the knockdown and overexpression of USP5. *P<0.05. USP5, ubiquitin-specific proteinase 5; sh, short hairpin; NC, negative control; OD, optical density.

Article Snippet: The human bladder urothelial carcinoma T24 cell line was purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Expressing, Knockdown, Over Expression, Light Microscopy, Western Blot, Ubiquitin Proteomics, Negative Control

Journal: Oncology Letters

Article Title: USP5 promotes tumor progression by stabilizing SLUG in bladder cancer

doi: 10.3892/ol.2024.14705

Figure Lengend Snippet: USP5 interacts with SLUG in human bladder cancer T24 cells. (A) Protein interaction network map of proteins bound to USP5 using data from the GeneMANIA database. (B) Colocalization of USP5 and SLUG in T24 cells was visualized using a confocal microscope, and ImageJ software was used for colocalization analysis of USP5 and SLUG expression. Pearson correlation analysis revealed that r > 0.5, and Manders' colocalization coefficient demonstrated that M1 > 0.5 and M2 > 0.5. (C) Computational docking model of USP5 and SLUG. (D) Endogenous and exogenous IP demonstrated the interaction of USP5 and SLUG in T24 cells. USP5, ubiquitin-specific proteinase 5; SLUG, Snail family zinc finger 2; IP, immunoprecipitation.

Article Snippet: The human bladder urothelial carcinoma T24 cell line was purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Microscopy, Software, Expressing, Ubiquitin Proteomics, Immunoprecipitation

Journal: Oncology Letters

Article Title: USP5 promotes tumor progression by stabilizing SLUG in bladder cancer

doi: 10.3892/ol.2024.14705

Figure Lengend Snippet: USP5 stabilizes SLUG by reducing its ubiquitination. (A) Protein expression and (B) mRNA levels of SLUG in the USP5 knockdown and overexpression T24 cell lines. (C) Remaining SLUG protein levels in the USP5 knockdown and overexpression T24 cell lines after treatment with CHX at several time points. (D) Western blotting revealed that USP5 regulated SLUG ubiquitination in T24 cells. SLUG proteins were isolated from the USP5 knockdown or overexpression T24 cells by Co-IP, and the ubiquitination of vimentin was then detected using western blotting. *P<0.05. USP5, ubiquitin-specific proteinase 5; SLUG, Snail family zinc finger 2; IP, immunoprecipitation; CHX, cyclohexanamide; sh, short hairpin; NC, negative control; UB, ubiquitination.

Article Snippet: The human bladder urothelial carcinoma T24 cell line was purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Ubiquitin Proteomics, Expressing, Knockdown, Over Expression, Western Blot, Isolation, Co-Immunoprecipitation Assay, Immunoprecipitation, Negative Control

Journal: Oncology Letters

Article Title: USP5 promotes tumor progression by stabilizing SLUG in bladder cancer

doi: 10.3892/ol.2024.14705

Figure Lengend Snippet: USP5 promotes the progression of bladder cancer by interacting with SLUG. (A) Protein expression and (B) mRNA levels of SLUG in the USP5 overexpression T24 cell lines with and without SLUG knockdown. Results of the (C) Transwell and (D) cell proliferation assays in the USP5 overexpression T24 cell line with or without SLUG knockdown. (E) Protein expression levels of epithelial-mesenchymal transition marker (E-cadherin, N-cadherin and vimentin) in the USP5 overexpression T24 cell lines with or without SLUG knockdown. *P<0.05; # P>0.05. USP5, ubiquitin-specific proteinase 5; SLUG, Snail family zinc finger 2; sh, short hairpin; OD, optical density.

Article Snippet: The human bladder urothelial carcinoma T24 cell line was purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Expressing, Over Expression, Knockdown, Marker, Ubiquitin Proteomics

Journal: Oncology Letters

Article Title: USP5 promotes tumor progression by stabilizing SLUG in bladder cancer

doi: 10.3892/ol.2024.14705

Figure Lengend Snippet: Pharmacological inhibition of USP5 suppresses bladder cancer progression. (A) Cell viability of T24 cells at different Degrasyn concentrations. (B) Protein expression and (C) mRNA levels of SLUG in the USP5 overexpression T24 cell line with or without Degrasyn treatment (USP5 inhibitor, 2 µg/ml). Results of the (D) Transwell and (E) cell proliferation assays in the USP5 overexpression T24 cell line with or without Degrasyn treatment (2 µg/ml). (F) Protein expression levels of epithelial-mesenchymal transition markers (E-cadherin, N-cadherin and vimentin) in the USP5 overexpression T24 cell line with or without Degrasyn treatment (2 µg/ml). *P<0.05; # P>0.05. USP5, ubiquitin-specific proteinase 5; SLUG, Snail family zinc finger 2; IC 50 , half maximal inhibitory concentration; OD, optical density.

Article Snippet: The human bladder urothelial carcinoma T24 cell line was purchased from Procell Life Science & Technology Co., Ltd.

Techniques: Inhibition, Expressing, Over Expression, Ubiquitin Proteomics, Concentration Assay